EC Medium (Granular Culture Media) For Determination of Fecal Coliforms And E. Coli Confirmatory Testing, 250g/bottle

EC Medium (Granular Culture Media)

Usages：
For determination of fecal coliforms and E. coli confirmatory testing.
 
Principle:
Tryptone provide carbon and nitrogen sources; the 3rd bile suppress gram-positive bacteria, especially against Gram-positive bacteria and fecal streptococci; lactose is fermentable sugars; potassium hydrogen phosphate and potassium dihydrogen phosphate as buffer ; sodium chloride maintains osmotic equilibrium.
 
Formulation (per liter):
Tryptone: 20.0g
The 3rd bile: 1.5g
Lactose: 5.0g
Dipotassium hydrogen: 4.0g
Potassium dihydrogen phosphate: 1.5g
Sodium chloride:5.0g
Final pH 6.9 ± 0.2
 
How to use:
1.Suspend 37g in 1 L of distilled water or deionized water, stirring heated to boiling until completely dissolved, packed in with a small test tube inverted tube, and autoclave at 121 ℃ for 15min, leave to cool to room temperature and set aside.
2.Diluted and treated sample.
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 
Specifications: 250g/bottle; 300ml*10bag/box 
 
References:
1. GB / T 4789.38-2008 People's Republic of China national standards for food safety standards of food hygiene microbiological testing E. coli count
2. GB / T 4789.39-2008 People's Republic of China national standards for food safety standards of food hygiene microbiological examination of fecal coliform count
3. SN 0169-92 People's Republic of China Import and Export Commodity Inspection industry standard export food coliform, fecal coliform and E. coli test methods
4 ISO7251-2005 miceobiology of food and animal feeding stuffs ----- Horizontal method for the detectcton and enumeration of presumptive Escherichia coli ---- Most probable number technique